Preservation of arachidonoyl phospholipids during tissue processing for electron microscopic autoradiography.
نویسندگان
چکیده
منابع مشابه
Electron Microscopic Autoradiography
An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H(3)-thymidine were examined electro...
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Iodide is concentrated from the blood by the thyroid gland; after oxidation it is incorporated in tyrosyl residues of the thyroglobulin molecule. Thus the halogen is stored as a component of thyroglobulin in the colloid of the thyroid follicle. Secretion of thyroid hormone is considered to require proteolysis of the thyroglobulin and release of iodinated thyronines from the base of the acinar c...
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Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...
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The fine structure of tritiated thymidine-labeled cells in antigen-stimulated mouse spleen germinal centers is described. In studies on the ultrastructural level, two labeled cell types found in germinal centers are observed. Large lymphocytes are characterized by their very numerous free ribosomes, a paucity of endoplasmic reticulum, relatively few mitochondria, and a poorly developed Golgi re...
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The feasibility of a technique for autoradiography of diffusible substances has been further tested by analysing the localization of steroids in rat testes with the light-and electron-microscope. Testes of rats were perfused with tritiated testosterone (3 min) followed by 15-min perfusion with buffer containing a 100-fold of unlabelled testosterone. Tissue samples were frozen, freeze dried, fix...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1985
ISSN: 0022-1554,1551-5044
DOI: 10.1177/33.8.3926867